» Proteomics Pilot Study [493 KB]
- Proteome Analysis of two groups (e.g. 3x control samples vs. 3x diseased or treated samples) by high resolution 2D electrophoresis with 2DE image analysis and statistical evaluation (Mann-Whitney-test, t-test, p<0.05).
» Protein identification [284 KB] by nanoLC-ESI-MSMS or MALDI-ToF-ToF-MS.
Protein identification by nanoLC-ESI-MSMS
Most sensitive and selective due to nanoLC-separation of peptides prior to MS/MS-analysis.
Provides mostly higher sequence coverage and valuable peptide information compared to
MALDI-ToF-ToF-MS. Includes tryptic digest and NCBInr protein database search.